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Dharmabunny, I need some help.

How do you remove a glutathione group from a molecule?
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Re: Dharmabunny, I need some help.

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    baconsmombaconsmom member
    5 Love Its First Answer First Anniversary Name Dropper
    edited December 2011
    Hire extra security.

    Call the non-emergency police number.

    Ask for the manager.

    No? Oh, well, then. 
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011
    WHO'S PAYING
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    ReturnOfKuusReturnOfKuus member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    *laughing helplessly*
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    AuntFloAuntFlo member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    I can't wait to hear the *real* answer.   I've always wanted to  know.
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    dharmabunnydharmabunny member
    First Anniversary 5 Love Its First Answer Name Dropper
    edited December 2011
    Crap, it's been so long since I've had to think about this stuff ;)

    I think glutathione-S-transferase (GST).  Works at the sulfur group.  Does that help?

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    ReturnOfKuusReturnOfKuus member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    That's what got it ON there in the first place, and it doesn't seem to be taking it off.  Crap, I have to eat up the whole GST and hope for the best, don't I?
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011
    this sounds like union bs
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    dharmabunnydharmabunny member
    First Anniversary 5 Love Its First Answer Name Dropper
    edited December 2011
    In Response to <a href="http://forums.theknot.com/Sites/theknot/Pages/Main.aspx/special-topic-wedding-boards_wedding-woes_dharmabunny-need?plckFindPostKey=Cat:Special%20Topic%20Wedding%20BoardsForum:47Discussion:53100a46-4fc8-44df-94ee-935b2fcaabc7Post:a02183c8-885b-4b47-b881-02d587fb3bbe">Re: Dharmabunny, I need some help.</a>:
    [QUOTE]That's what got it ON there in the first place, and it doesn't seem to be taking it off.  Crap, I have to eat up the whole GST and hope for the best, don't I?
    Posted by ReturnOfKuus[/QUOTE]

    Hold up.  Are you working with a fusion protein?  GST-something or other?
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011
    WAIT -- IF NEITHER OF YOU KNOW WHAT YOU ARE DOING, BACK AWAY.


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    ReturnOfKuusReturnOfKuus member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    No, nothing like that.  I'm trying to retrieve stuff that has been conjugated for excretion out of the urine, and while I can get back the stuff that has been sulfated or glucuronidated, the glutathione conjugates are a problem.
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011

    first poo problems, and now pee problems.  kuus, you need more fiber and water in your diet.  4realz.

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    ReturnOfKuusReturnOfKuus member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    I don't know if it would sound better or worse if I told you that it isn't my own pee.
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    edited December 2011
    Ded @ hmo
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011
    do you know where it came from, or is it just something you found on the street?
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    dharmabunnydharmabunny member
    First Anniversary 5 Love Its First Answer Name Dropper
    edited December 2011
    In Response to <a href="http://forums.theknot.com/Sites/theknot/Pages/Main.aspx/special-topic-wedding-boards_wedding-woes_dharmabunny-need?plckFindPostKey=Cat:Special%20Topic%20Wedding%20BoardsForum:47Discussion:53100a46-4fc8-44df-94ee-935b2fcaabc7Post:7b67ebd1-ad8c-408a-a114-b578fe8ab8ff">Re: Dharmabunny, I need some help.</a>:
    [QUOTE]No, nothing like that.  I'm trying to retrieve stuff that has been conjugated for excretion out of the urine, and while I can get back the stuff that has been sulfated or glucuronidated, the glutathione conjugates are a problem.
    Posted by ReturnOfKuus[/QUOTE]


    Ahh, got it.  A little out of my expertise (my mian GST dealings were fusion protein based), but you may need to assault it with GST.  GST never seemed to be a super robust enzyme.  Maybe you could play around with pH or something of that sort, or do a little googling or asking around to see what others did to goose up the efficiency of GST purifications.

    Or nuke it, like hmo's suggestion
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    ReturnOfKuusReturnOfKuus member
    First Anniversary 5 Love Its First Comment Name Dropper
    edited December 2011
    Well, it is kind of my pee.  It's synthetic pee.  I made it myself from various extracts.
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    hmonkeyhmonkey member
    First Anniversary 5 Love Its Name Dropper First Comment
    edited December 2011
    kuus + dharmabunny = 2 girls, 1 cup
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    mrsconn23mrsconn23 member
    First Anniversary First Answer 5 Love Its First Comment
    edited December 2011
    Hmo-you're killing me. 
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    edited December 2011
    i love hmo x infinity
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    LnR70707LnR70707 member
    First Anniversary 5 Love Its First Comment
    edited December 2011
    DED @ hmo
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