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Dharmabunny, I need some help.

ReturnOfKuusReturnOfKuus member
Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
in Wedding Woes
How do you remove a glutathione group from a molecule?
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Re: Dharmabunny, I need some help.

  • baconsmombaconsmom member
    Knottie Warrior 5000 Comments 500 Love Its First Answer
    edited December 2011
    Hire extra security.

    Call the non-emergency police number.

    Ask for the manager.

    No? Oh, well, then. 
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  • hmonkeyhmonkey member
    Ninth Anniversary 10000 Comments 500 Love Its Name Dropper
    edited December 2011
    WHO'S PAYING
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  • ReturnOfKuusReturnOfKuus member
    Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    *laughing helplessly*
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  • AuntFloAuntFlo member
    Tenth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    I can't wait to hear the *real* answer.   I've always wanted to  know.
    �
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  • dharmabunnydharmabunny member
    Ninth Anniversary 500 Love Its 1000 Comments First Answer
    edited December 2011
    Crap, it's been so long since I've had to think about this stuff ;)

    I think glutathione-S-transferase (GST).  Works at the sulfur group.  Does that help?

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  • ReturnOfKuusReturnOfKuus member
    Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    That's what got it ON there in the first place, and it doesn't seem to be taking it off.  Crap, I have to eat up the whole GST and hope for the best, don't I?
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  • hmonkeyhmonkey member
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    edited December 2011
    this sounds like union bs
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  • dharmabunnydharmabunny member
    Ninth Anniversary 500 Love Its 1000 Comments First Answer
    edited December 2011
    In Response to <a href="http://forums.theknot.com/Sites/theknot/Pages/Main.aspx/special-topic-wedding-boards_wedding-woes_dharmabunny-need?plckFindPostKey=Cat:Special%20Topic%20Wedding%20BoardsForum:47Discussion:53100a46-4fc8-44df-94ee-935b2fcaabc7Post:a02183c8-885b-4b47-b881-02d587fb3bbe">Re: Dharmabunny, I need some help.</a>:
    [QUOTE]That's what got it ON there in the first place, and it doesn't seem to be taking it off.  Crap, I have to eat up the whole GST and hope for the best, don't I?
    Posted by ReturnOfKuus[/QUOTE]

    Hold up.  Are you working with a fusion protein?  GST-something or other?
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  • hmonkeyhmonkey member
    Ninth Anniversary 10000 Comments 500 Love Its Name Dropper
    edited December 2011
    WAIT -- IF NEITHER OF YOU KNOW WHAT YOU ARE DOING, BACK AWAY.


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  • ReturnOfKuusReturnOfKuus member
    Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    No, nothing like that.  I'm trying to retrieve stuff that has been conjugated for excretion out of the urine, and while I can get back the stuff that has been sulfated or glucuronidated, the glutathione conjugates are a problem.
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  • hmonkeyhmonkey member
    Ninth Anniversary 10000 Comments 500 Love Its Name Dropper
    edited December 2011

    first poo problems, and now pee problems.  kuus, you need more fiber and water in your diet.  4realz.

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  • ReturnOfKuusReturnOfKuus member
    Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    I don't know if it would sound better or worse if I told you that it isn't my own pee.
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  • [Deleted User][Deleted User] member
    Ninth Anniversary 1000 Comments 25 Love Its Name Dropper
    edited December 2011
    Ded @ hmo
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  • hmonkeyhmonkey member
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    edited December 2011
    do you know where it came from, or is it just something you found on the street?
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  • dharmabunnydharmabunny member
    Ninth Anniversary 500 Love Its 1000 Comments First Answer
    edited December 2011
    In Response to <a href="http://forums.theknot.com/Sites/theknot/Pages/Main.aspx/special-topic-wedding-boards_wedding-woes_dharmabunny-need?plckFindPostKey=Cat:Special%20Topic%20Wedding%20BoardsForum:47Discussion:53100a46-4fc8-44df-94ee-935b2fcaabc7Post:7b67ebd1-ad8c-408a-a114-b578fe8ab8ff">Re: Dharmabunny, I need some help.</a>:
    [QUOTE]No, nothing like that.  I'm trying to retrieve stuff that has been conjugated for excretion out of the urine, and while I can get back the stuff that has been sulfated or glucuronidated, the glutathione conjugates are a problem.
    Posted by ReturnOfKuus[/QUOTE]


    Ahh, got it.  A little out of my expertise (my mian GST dealings were fusion protein based), but you may need to assault it with GST.  GST never seemed to be a super robust enzyme.  Maybe you could play around with pH or something of that sort, or do a little googling or asking around to see what others did to goose up the efficiency of GST purifications.

    Or nuke it, like hmo's suggestion
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  • ReturnOfKuusReturnOfKuus member
    Eighth Anniversary 5000 Comments 500 Love Its Name Dropper
    edited December 2011
    Well, it is kind of my pee.  It's synthetic pee.  I made it myself from various extracts.
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  • hmonkeyhmonkey member
    Ninth Anniversary 10000 Comments 500 Love Its Name Dropper
    edited December 2011
    kuus + dharmabunny = 2 girls, 1 cup
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  • mrsconn23mrsconn23 member
    Knottie Warrior 10000 Comments 500 Love Its First Answer
    edited December 2011
    Hmo-you're killing me. 
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  • MinMMinM
    10000 Comments
    edited December 2011
    i love hmo x infinity
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  • LnR70707LnR70707 member
    Tenth Anniversary 2500 Comments 5 Love Its
    edited December 2011
    DED @ hmo
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